Qualitative and Quantitative Investigations of Amino Acids Content from the Flowers of Moringa olifera Lam. (Dan-da-lun)

In the present research work, one of Myanmar well-known medicinal plants, Moringa olifera Lam., Myanmar named Dan-da-lun was selected for chemical investigations. Firstly, the preliminary phytochemical screenings of selected sample were carried out. Furthermore, mineral contents of selected sample were determined by Atomic Absorption Spectrometry (AAS) method. Moreover, qualitative tests of amino acids were examined. In addition, the qualitative determination of total amino acid content in selected plant materials were estimated by one and two dimensional Paper Chromatography methods and Thin Layer Chromatography methods by using the color developer ninhydrin reagent and identified with standard amino acids. Finally, the quantitative determination of amino acid content was determined by using two dimensional Paper Chromatography, ninhydrin assay method and UV visible spectrophotometer. The content percentages of some amino acids in flowers of Dan-da-lun were calculated as Arginine 0.8%, Aspartic acid 1.6%, Glycine 2.64%, Lysine 2.0%, Threonin 0.6%, and Valine 0.6% by applying the various concentration absorbance curves of standard six amino acids