Androgenesis of Selected Indica Rice (Oryza sativa L.) Genotypes and F1 Hybrids

This experiment was carried out to evaluate the callusing ability and green plant regeneration of selected indica rice genotypes and F1 progenies after crossing . Shwe Man-1, Sin Akari-3, Sin Thwe Latt, Thee Htat Yin and Yar-8 genotypes were selected as parental lines based on callusing ability. Five crosses were obtained by crossing the selected genotypes. Anthers of selected indica rice genotypes and F1 hybrids were cultured in N6 medium supplemented with 2.0 mg•L-1 2,4-Dichloro phenoxyacetic acid (2,4-D) and 0.5 mg•L-1 kinetin for callus induction. Calli were transferred onto MS medium supplemented with 1 mg.L-1 Naphthalene acetic acid (NAA), 1 mg.L-1 Indole 3-acetic acid (IAA), 1 mg.L-1 6-Benzylaminopurine (BAP) and 2.0 mg•L-1 kinetin. All tested genotypes showed callus formation except one cross (Sin Thwe Latt x Sin Akari-3) that showed no response on anther culture. The callus induction percentage of response genotypes was 0.1-2.6 %. Green plant formation was 6.3-37.0 %. Among parents, Yar-8 and Shwe Man-1 gave the highest response on callus induction and green plant formation. Cross of Yar-8 x Thee Htat Yin increased callusing and green plant formation. The anther-derived plants of these three genotypes were haploid and double haploids. Therefore, these genotypes have the potential in double haploid production through anther culture in rice breeding program