Production of Lactic Acid and lysine from Cassava Starch and Cassava Waste

The present research deals with the production of Lactic acid and lysine from Cassava Starch and Cassava Waste. Firstly the starch (10.5% yields) was extracted from cassava waste (pulp) and it was then converted to glucose syrup by enzymatic hydrolysis using α-amylase and aminoglucosidase enzymes. The concentration of glucose syrup (91.40% yield, based on raw cassava starch), (9% yield, based on cassava waste) could be determined as 3.02 mg in the prepared 5 mg/mL solution From cheese, Lactobacillus caseiwas isolated and identified, and it was applied for the fermentation of cassava glucose syrup at pH 5.5 and 8% inoculum level for 27h. The lactic acid was produced with different concentration of glucose syrup (2%, 4%, 6%, 8%, and10 %) at selected optimum conditions. The highest lactic acid content (0.045 g/mL) and highest yield productivity (1.125 g/mL of based on glucose syrup) were observed by using 4% of cassava glucose syrup, On the other hand, Corynebacteriim glutamicum was isolated from soil, and lysine was produced from the fermentation of glucose syrup with C. glutamicum. The highest lactic acid content (0.025 g/mL) and highest yield productivity (0.227 g/mL) based on glucose syrup were obtained