1. An apparatus for the simultaneous measurements of volume change, fluorescence intensity of pyridine nucleotides and oxygen consumption of mitochondria has been constructed. 2. Oxygen consumption is measured by the rotating platinum electrode with a modification of Hagihara's system, attached in a cuvette of the apparatus. 3. Volume changes of mitochondria (swelling-shrinkage) are measured by the 90° light-scattering at 650 mμ. 4. Relative fluorescence intensity of pyridine nucleotides is measured by the fluorometer: for the excitation, a bright light at 365 mμ. line of mercury lamp is isolated through the filter and exposed to the mitochondria suspended in a cuvette of the apparatus, and fluorescent emission is analyzed by a gr...
AbstractA modified version of fluorescence correlation spectroscopy (FCS) closely related to the pho...
An apparatus is described with which it is possible to measure precisely fluorescence spectra, not o...
An integrated instrumentation which combines in the same system static and dynamic laser lightscatte...
An apparatus for simultaneous measurement of 90° light scattering, pyridine nucleotide fluorescence ...
1. An apparatus for the simultaneous measurements of volume change, fluorescence intensity of pyridi...
Mitochondria are cell organelles that have a crucial role in cellular energy metabolism. Mitochondri...
In the last ten years, the use of fluorescent probes developed to measure oxygen has resulted in sev...
Abstract A device for optical biopsy with a fluorescence spectroscopy channel and a fine-needle opt...
The overall aim of this study was verification of the possibility to register the change of NADH flu...
<p>(<b>A</b>) In the FSC/SSC plot of the isolated mitochondria, 10000 events were collected within g...
An integrated instrumentation which combines in the same system static and dynamic laser light scatt...
<p>Our experimental set-up was composed of an oxygraph, a spectrophotometer and a luminometer. An op...
Background and objectives: Mitochondria require oxygen to generate energy for essential cellular pro...
Photobleaching of the fluorophore fluorescein in an aqueous solution is dependent on the oxygen conc...
Oxidation-reduction chemistry is fundamental to the metabolism of all living organisms, and hence qu...
AbstractA modified version of fluorescence correlation spectroscopy (FCS) closely related to the pho...
An apparatus is described with which it is possible to measure precisely fluorescence spectra, not o...
An integrated instrumentation which combines in the same system static and dynamic laser lightscatte...
An apparatus for simultaneous measurement of 90° light scattering, pyridine nucleotide fluorescence ...
1. An apparatus for the simultaneous measurements of volume change, fluorescence intensity of pyridi...
Mitochondria are cell organelles that have a crucial role in cellular energy metabolism. Mitochondri...
In the last ten years, the use of fluorescent probes developed to measure oxygen has resulted in sev...
Abstract A device for optical biopsy with a fluorescence spectroscopy channel and a fine-needle opt...
The overall aim of this study was verification of the possibility to register the change of NADH flu...
<p>(<b>A</b>) In the FSC/SSC plot of the isolated mitochondria, 10000 events were collected within g...
An integrated instrumentation which combines in the same system static and dynamic laser light scatt...
<p>Our experimental set-up was composed of an oxygraph, a spectrophotometer and a luminometer. An op...
Background and objectives: Mitochondria require oxygen to generate energy for essential cellular pro...
Photobleaching of the fluorophore fluorescein in an aqueous solution is dependent on the oxygen conc...
Oxidation-reduction chemistry is fundamental to the metabolism of all living organisms, and hence qu...
AbstractA modified version of fluorescence correlation spectroscopy (FCS) closely related to the pho...
An apparatus is described with which it is possible to measure precisely fluorescence spectra, not o...
An integrated instrumentation which combines in the same system static and dynamic laser lightscatte...