Infeluences of adrenalin, cortisone and ACTH on eosinophils in bone marrow tissue culture (With some sug­gestions for examining the anterior pituitary function)

1. Adrenalin, when acting directly on eosinophils, brings about a diminution in the wandering velocity of eosinophils but it has no influence on the number of the cells. Judging from the movement patterns of eosinophils this drug acts as to impede the motive function. 2. Acting directly on eosinophils, cortisone markedly decreases the wandering velocity of these cells and also brings about the diminution in the number of the cells. Likewise from the movement patterns of eosinophils, this drug markedly impedes the motive function of the cells. 3. ACTH (adrenocorticotropic hormone), when acting directly on eosinophils, enhances the wandering velocity of eosinophils but it in no way affects the number of eosinophils. Judging from the eosinophil movement patterns, this drug markedly promotes the motive function. 4. Although adrenalin brings about a decrease in the number of peripheral eosinophils in hypophysectomized dogs, the rate of the decrease is less than that observable in the case of normal dogs. 5. Cortisone brings about no significant change in the number of peripheral eosinophils in hypophysectomized dogs, but is induces a decrease in peripheral eosinophils of normal dogs. 6. ACTH acts as to decrease the number of peripheral eosinophils to an equal. degree in both hypophysectomized and normal dogs. 7. When cortisone is administered simultaneously with Adrex, a marked decrease in peripheral eosinophils is brought about in hypophysectomized dogs. 8. By means of the bone-marrow tissue culture of hypophysectomized dogs it has been confirmed that the blood plasma of hypophysectomized dogs lacks an essential factor for cortisone to induce eosinopenia in perpheral blood. 9. The decrease in eosinophils of peripheral blood induced by cortisone has been proved to be dependent upon the presence or absence of the pituitary body. 10. Taking the decrease in peripheral eosinophils by cortisone administration as the criterion, the author has carried out clinical observations with this method and obtained anticipated results.