Studies on the organellae of liver of cancer bearing animals II. Catalase activity in the liver of animals treated with cyto-plasmic organellae from hepatoma cell (AH 130) and liver cell of the hepatoma bearing animals

The author studied the distribution of polysaccharides and the amino-acid composition of cytoplasmic organellae, the problems that have come to call a great interest in the field of studies on cancer bearing animals. And also biochemical and electron microscopic observations were carried out to study the influences of cytoplasmic organellae in the cancer cells (AH 130), the livers of cancer bearing animals, and normal liver on the catalase activity of the liver. The results obtained are as follows : Cytoplasmic organellae of various cells do not affect so markedly the hexose metabolism of the liver. As for the amino-acid pattern of cytoplasmic organellae of various cells studied by paperchromatography, it is interesting to note that the pattern of the liver of cancer bearing animals, shows lack in histidine, while in can~er tissue and in the liver of cancer bearing animals an increase in phenylalanine can be observed. The decrease in the liver catalase activity is caused by the primary factor of cancer cells, especially their microsomes, and also by the secondary factor of the liver mitochondria in cancer bearing animals. On the other hand, the mitochondria of cancer cells, instead of reducing the catalase activity in the liver, markedly increases the catalase activity. By the morphological changes observed with light microscope and electron microscope, liver cells revealed marked morphological differences, proving that the microsomes of hepatoma cell induce considerably marked changes in the liver, while the mitochondria of hepatoma cell, on the contrary, induce the hypertrophy of liver cells. Sirriilarly in the electron microscopic observations the mitochondria of mouse liver injected with cancer mitochondria are enlarged, but no destruction of cellular structures such as cristae can be recognized. Also microbodies and the growing process of mitochondria can be observed, but no marked changes in endoplasmic reticulum.