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Not AvailableSoluble seed protein of different Citrus species was compared on discontinuous electrophoretic system. Five polyembryonic and three monoembryonic species with elite ecotypes formed 31 distinct, unambiguous bands in the range of 4 to 71 kD with four conserved bands in all the ecotypes irrespective of species with average molecular weight of 4, 10, 20, 35 KD, respectively. The major storage protein globulin showed a conserved nature without having any differential expression relevant to species or ecotype marking. A 46 kilo Dalton band was unique to monoembryonic types. Protein profile reveals close association of C. reticulata and C. sinensis indicating a probable co-evolution. Hierarchical Cluster analysis showed the monoembryonic types in a single cluster while in polyembryonic types C. reticulata paired with C. sinensis while C. limon and C. aurantifolia came together. The closeness of two polyembryonic species C. reticulata and C. sinensis with the monoembryonates suggested evolution of the previous types from the later or by hybridization. Study revealed the uniqueness of Citrus grandis among monoembryonates. C. papedia showed altogether different protein \ profile with some homology with C. aurantifolia. The study confirms the role of cross breeding and mutation followed by natural selection in co evolution of different ecotypes.Not Availabl