Background: There has been an increasing interest in the association between ambient temperature and violence and crime, in the context of global warming. We aimed to evaluate the association between daily ambient temperature and intentional homicide—a proxy for overall inter-personal violence. Methods: We collected daily weather and crime data from 9 large US cities (Chicago, Detroit, Fort Worth, Kansas City, Los Angeles, Louisville, New York, Tucson and Virginia Beach) from 2007 to 2017. A time-stratified case-crossover design was used. The associations were quantified by conditional logistic regression with distributed lag models, adjusting for relative humidity, precipitation and effects of public holidays. City-specific odds ratios (OR...