Survey of aflatoxin residue in feed and milk samples in Kirikkale province, Turkey

WOS: 000425921400013In this study total aflatoxin (AF) and aflatoxin M-1 (AFM(1)) contamination in feed and milk samples obtained from the dairy cow farms in Kirikkale region were studied. A total of 154 dairy cow feed and 154 raw milk samples were obtained from the villages (Delice, Keskin, Sulakyurt, Bahsili, Yahsihan, Celebi, Karakecili, Baliseyh) and central of Kirikkale province, Turkey, between the years June 2012-August 2013. The quantitative analysis of total AF and AFM(1) in the collected samples were carried out using an enzyme-linked-immunoassay (ELISA) with commercial kits (HELICA biosystems inc.). Total AF was detected in all feed samples and also AFM(1) contamination was found in all of the milk samples. In 5 of 154 feed samples the total AF's were above 20 mu g/kg. In none of the milk samples AF was above the legal limit. The mean AF residue level for concentrated feed was 6.43 +/- 7.01 mu g/kg, and ranged from 0.20 mu g/kg to 28.80 mu g/kg. The mean AFM(1) residue for milk samples was 1.73 +/- 2.20 ng/L, and ranged from 0.08 ng/L to 10.11 ng/L. In conclusion although all of the milk samples were contaminated with AFM(1), the amounts were within the legal limits that are allowed in milk. On the other hand in 3.25% of the feed samples, total AF was above 20 mu g/kg. The occurrence of AFM(1) may not be considered as a possible risk for public health. Strategies and monitoring programs to prevent aflatoxin contamination is recommended.Kirikkale University SRP Coordination Unit [2011/41]This study is supported by the Kirikkale University SRP Coordination Unit Project No: 2011/41