Effects of Acute and Chronic Electroconvulsive Shocks on Glycogen Synthase Kinase 3β Level and Phosphorylation in Mice

WOS: 000330362600012PubMed: 23807397Objectives Glycogen synthase kinase 3 beta (GSK-3 beta) is recently proposed as a novel target in the treatment of mood disorders. Recent evidence has suggested that acute and chronic administration of antidepressants led to inhibition of GSK-3 beta via phosphorylation of Serine9 residue. Acute electroconvulsive shock (ECS) has been reported to increase GSK-3 beta phosphorylation transiently. In this study, the changes in the level of GSK-3 beta and its phoshorylated form (phospho-Ser9-GSK-3 beta) following chronic ECS (cECS) were investigated in mice. Methods Mice were given daily ECS via bilateral corneal electrodes for 10 consecutive days in the chronic group and a single ECS in the acute group. Electrodes were applied without stimulation in corresponding sham groups. Immunoblotting for GSK-3 beta and phospho-Ser9-GSK-3 beta was performed with the frontal cortex and hippocampus samples, extracted 10 minutes after single ECS, and 24 hours after the last ECS in the chronic group. The optical densities of the bands obtained were compared between the active treatment and sham groups for each condition separately. Results The level of phospho-Ser9-GSK-3 beta was not different following chronic ECS, but significantly higher following acute ECS, compared with the corresponding sham group, in the hippocampus and frontal cortex. The level of GSK-3 beta was similar to sham following both acute and chronic ECS, in both regions. Conclusions The transient increase in GSK-3 beta phosphorylation observed following acute ECS in the mice hippocampus and frontal cortex was not found to persist 24 hours following chronic ECS. The mechanism of action of ECS does not seem to involve persistent change in the level and phosphorylation of GSK-3 beta.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [SBAG-HD-18-105S012]; Hacettepe University (Scientific Research and Development Office)Hacettepe University [04D10101004]; Brain Research Society (Turkey)This study was financially supported by the Scientific and Technological Research Council of Turkey (TUBITAK, grant no. SBAG-HD-18-105S012), Hacettepe University (Scientific Research and Development Office, grant number: 04D10101004), Brain Research Society (Turkey, 2004 Project Support Award)