Colonization, translocation and antibody response in experimental Campylobacter infection of chickens

YARDIMCI, Hakan/0000-0002-5994-5792; Akan, Mehmet/0000-0002-7342-1450WOS: 000180152200025In this study we examined factors affecting the colonization of campylobacters in chick intestines, colonization times and elicited immune responses. In chicks, infected orally by minimal infective dose (100) (MID100), colonization in the intestines was observed to reach 100% during the 3-week period following C. jejuni and C. coli inoculation. When the SDS-PAGE protein profiles of the inoculated and isolated strains were examined similarities of 98.3% among C. jejuni strains and 98.5% among C. coli strains were determined. The results obtained from the ELISA tests performed with blood sera in the group infected with C. jejuni and in the control group for the 2nd, 3rd and 4th weeks were 0.27-0.25, 0.22-0.18 and 0.27-0.17, and the group infected with C. coli and the control group were 0.28-0.25, 0.24-0.19 and 0.32-0.18. After the immunoblotting analyses, specific bands that have molecular masses of 61, 47.5, 29.8 and 25.6 kDa were identified from C. jejuni strains and 62.5, 48 ve 31 kDa from C. coli strains, respectively