Enzyme-controlled sensing-actuating nanomachine based on Janus Au-mesoporous silica nanoparticles

[EN] Novel Janus nanoparticles with Au and mesoporous silica faces on opposite sides were prepared using a Pickering emulsion template with paraffin wax as the oil phase. These anisotropic colloids were employed as integrated sensing-actuating nanomachines for enzyme-controlled stimuli-responsive cargo delivery. As a proof of concept, we demonstrated the successful use of the Janus colloids for controlled delivery of tris(2,2'-bipyridyl) ruthenium(II) chloride from the mesoporous silica face, which was grafted with pH-sensitive gatelike scaffoldings. The release was mediated by the on-demand catalytic decomposition of urea by urease, which was covalently immobilized on the Au face.R. V. acknowledges a Ramon & Cajal contract from the Spanish Ministry of Science and Innovation. Financial support from the Spanish Ministry of Science and Innovation CTQ2011-24355, CTQ2009-12650, CTQ2009-09351, MAT2009-14564-C04-01, MAT2012-38429-C04-01 and Comunidad de Madrid S2009/PPQ-1642, programme AVANSENS, is gratefully acknowledged. The Generalitat Valencia (project PROMETEO/2009/016) is also acknowledged.Villalonga, R.; Díez, P.; Sánchez, A.; Aznar, E.; Martínez-Máñez, R.; Pingarrón, J. (2013). Enzyme-controlled sensing-actuating nanomachine based on Janus Au-mesoporous silica nanoparticles. Chemistry - A European Journal. 19(24):7889-7894. https://doi.org/10.1002/chem.201300723788978941924Perro, A., Reculusa, S., Ravaine, S., Bourgeat-Lami, E., & Duguet, E. (2005). Design and synthesis of Janus micro- and nanoparticles. Journal of Materials Chemistry, 15(35-36), 3745. doi:10.1039/b505099eJiang, S., Chen, Q., Tripathy, M., Luijten, E., Schweizer, K. S., & Granick, S. (2010). Janus Particle Synthesis and Assembly. 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