Triplet excited states as chiral reporter for the binding of drugs to transport proteins

[EN] The triplet excited state of flurbiprofen methyl ester (FBPMe) has been used as a chiral reporter for the two binding sites of human serum albumin (HSA). The occupation level of the binding sites has been estimated from regression analysis of the triplet decays at several [FBPMe]/[HSA] ratios. The data agree with two high affinity binding sites (I and II) that are populated to a different extent. A remarkable stereodifferentiation has been found in the drug triplet lifetimes within the protein microenvironment.The UPV (Grant PI 2003-0522 and fellowship to I.V.), the MEC (Grant CTQ2004-03811), and Generalitat Valenciana (Grant GV2004-0536 and Grupos03/082) are gratefully acknowledged for financial support.Jiménez Molero, MC.; Miranda Alonso, MÁ.; Vayá Pérez, I. (2005). Triplet excited states as chiral reporter for the binding of drugs to transport proteins. Journal of the American Chemical Society. 127(29):10134-10135. https://doi.org/10.1021/ja051448910134101351272