Genotoxic damages in zebrafish submitted to a polymetallic gradient displayed by the Lot River (France)
- Orieux, N.
- Cambier, S.
- Gonzalez, P.
- Morin, B.
- Adam, C.
- Garnier-Laplace, J.
- Bourdineaud, J.-P.
DOIAbstract
Genotoxic effects of a polymetallic pollution gradient displayed by the Lot River and one of its tributary have been assessed on zebrafish Danio rerio. Three methods were compared: RAPD-PCR, the comet assay, and 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) formation. The fishes were exposed for 14 days to waters collected from three stations: Joanis, a site polluted by cadmium (Cd) and zinc (Zn) (mean concentrations: 15. γg Cd/L and 550. γg Zn/L), Bouillac (mean concentrations: 0.55. γg Cd/L and 80. γg Zn/L), and Boisse-Penchot, a reference station (mean concentrations:<0.05. γg Cd/L and 7. γg Zn/L). The quantitative RAPD-PCR methodology proved to be sensitive enough to unmask metal genotoxicity after 3 and 7 days of exposure to Joanis wa...
Add to ORKG