Capacity of two Staphylococcus aureus strains with different adaptation genotypes to persist and induce damage in bovine mammary epithelial cells and to activate macrophages

Sacco, Sofía Clara
Velázquez, Natalia S.
Renna, María Sol
Beccaria, Camila
Baravalle, Celina
Pereyra, Elizabet Amanda Lorena
Monecke, Stefan
Calvinho, Luis Fernando
Dallard, Bibiana Elisabet

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Publication date

January 2020

DOI

10.1016/j.micpath.2020.104017

Publisher

Elsevier BV

Journal

Microbial Pathogenesis

Language

English

Abstract

The aim of this study was to evaluate and compare the ability to adhere/internalize, persist, and induce damage in mammary epithelial cells (MAC-T) of two Staphylococcus aureus strains with different adaptation genotypes (low and high) to the bovine mammary gland (MG). Also, the phagocytic and bactericidal capacity induced after the interaction between macrophages, isolated from mammary secretion, of both S. aureus strains was evaluated. Two isolates (designated 806 and 5011) from bovine intramammary infection (IMI) harboring genes involved in adherence and biofilm production, belonging to different capsular polysaccharide (CP) type, accessory gene regulator (agr) group, pulsotype (PT) and sequence type/clonal complex (ST/CC). Strains 806 a...