Detection of Apoptosis in Cancer Cells Using Heat Shock Protein 70 and p53 Antibody Conjugated Quantum Dot Nanoparticles
- Matyushkin, Lev B.
- Aleksandrova, Olga A.
- Drobintseva, Anna O.
- Kvetnoy, Igor M.
- Krylova, Yuliya S.
- Marchenko, Yaroslav Y.
- Mazing, Dmitriy S.
- Moshnikov, Vyacheslav A.
- Мusikhin, Sergey F.
- Nikolaev, Boris P.
- Polyakova, Victoriya O.
- Shevtsov, Maxim A.
- Yakovleva, Ludmila Y.
DOIAbstract
Clinical experience indicates that enhanced level of heat shock protein 70 (Hsp70) and p53 correlates with poor prognosis due to malignant cell overexpression of these proteins in tumor progression. Cadmium selenide quantum dots (QDs) were synthesized in aqueous solution using mercaptopropionic acid and L-cysteine (L-Cys) as ligands. They were conjugated with a monoclonal antibody (Ab) to p53 and cmHp70.1 to Hsp70 for detection of cancer cell apoptosis that was demonstrated in the experiment by fluorescent confocal microscopy both for breast carcinoma cells and for thyroid tissue. It is shown that in comparison with organic dyes, quantum dots have superior photostability of tracking apoptosis in cancer cells for longer time
Add to ORKG