Antifungal activity of essential oils against fungus Rhizoctonia solani

Cilj je ovog rada utvrditi kakav su utjecaj eterična ulja imala na porast micelija gljive Rhizoctonia solani i koja su eterična ulja i pri kojoj količini imala antifungalan učinak. Radilo se sa sljedećim eteričnim uljima: anis, bor, cimet kora, citronela, čajevac, čempres, eukaliptus, klinčić, lavanda, naranča slatka, ružmarin i timijan. Ulja su se primjenjivala u količinama od 5, 10, 15, 25 i 50 µl, a zona inhibicije mjerila se četvrti i sedmi dan. Učinci dvanaest eteričnih ulja na gljivu Rhizoctonia solani rađeni su u uvjetima in vitro. Najjači antifungalan učinak imalo je eterično ulje timijana i anisa koje je izuzetno inhibiralo rast micelija i s primjenom u najmanjoj količini. Uz eterično ulje timijana i anisa jak utjecaj imala su i ulja lavande, citronele i čajevca. Najslabije antifungalno djelovanje imalo je eterično ulje eukaliptusa, bora i naranče slatke koja pri određenim količinama nisu ni imala antifungalni učinak.The aim of this study was to determine what kind of effect did the essential oils have on the mycelial growth of the fungus Rhizoctonia solani, which essential oils and at what quantity had an antifungal effect. The study was done with the following essential oils: anise, pine, cinnamon bark, citronella, tea tree, cypress, eucalyptus, clove, lavender, sweet orange, rosemary and thyme. Oils were applied in quantities of 5, 10, 15, 25 and 50 µL, and the zone of inhibition was measured on the fourth and the seventh day of incubation. The effects of twelve essential oils on the fungus Rhizoctonia solani were performed in vitro. The strongest antifugal effect had the essential oil of thyme and anise which completely inhibited the mycelial growth even in the smallest amount of application. Along with the essential oil of thyme and anise, the lavender, citronella and tea tree oils also had a strong impact. The weakest antifungal effect had the essential oil of eucalyptus, pine and sweet orange, which in certain quantities did not even have an antifungal effect