Standardization procedure for fow cytometry data harmonization in prospective multicenter studies

One of the most challenging objective for clinical cytometry in prospective multicenter immunomonitoring trials is to compare frequencies, absolute numbers of leukocyte populations and further the mean fuorescence intensities of cell markers, especially when the data are generated from diferent instruments. Here, we describe an innovative standardization workfow to compare all data to carry out any large-scale, prospective multicentric fow cytometry analysis whatever the duration, the number or type of instruments required for the realization of such projects.Innovative Medicines Initiative Joint Undertaking 115565French National Research Agency (ANR) ANR-11-LABX-0016-001Region BretagneEuropean Union (EU)EFPIA companies