Vulnerability of reef-building corals towards global change

Global warming is leading to large-scale coral bleaching and mass mortality, but also to increases in tropical storms' frequency and intensity. Storms allow fragmentation of reef-building corals and can lead to near-shore salinity reduction which, combined with ocean warming, will aggravate coral distress. In order to assess the susceptibility of different coral species to these environmental stressors, small fragments of nine coral species of the Indo-Pacific region were exposed to different thermal (26°C, 30°C, 32°C) and hyposaline (26°C-33psu, 30°C-33psu, 26°C-20psu, 30°C-20psu) experimental treatments for 60 days. Several parameters were assessed at different levels of biological organization: at the organism level (total and partial mortality, and coral condition based in bleaching levels), physiological level (growth rate and regeneration rate of artificially inflicted lesions), and molecular level (superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), and lipid peroxidation (LPO)). Also, in order to test two different approaches to be applied in the monitoring of the effects of heat stress, some parameters were combined in integrated biomarker response indices, either in a molecular approach, approach A, using GST, CAT, LPO, and SOD, or in an approach that integrates the molecular, physiological and organism levels, approach B, using GST, CAT, LPO, SOD, partial mortality, and growth rate. Results indicate that Pocillopora damicornis and Stylophora pistillata were the most vulnerable at 30°C. Psammocora contigua, Turbinaria reniformis, and Galaxea fascicularis were the most tolerant species at 32°C. The species P. contigua and G. fascicularis were the most tolerant to low salinity (26°C-20psu). The species G. fascicularis was the only one capable of surviving the combined effect of high temperature and low salinity (30°C-20psu). Approach B, the most integrative approach, was considered the most adequate for evaluating the health of reef corals since it better discriminated the stress suffered by the tested species.MAREUCIBI