Similarity Check ANACTINOMYCETES (ISOLATE T34) AS ANTIBIOTIC PRODUCER AGAINTS Staphylococcus aureus AND BIOAUTOGRAPHY ANALYSIS

Background. Actinomycetes is a group of microorganisms producing many kinds of active compounds. One of them is antibiotic. Objective. The purpose of this study is to determine the potency of Actinomycetes (isolate T34)as antibioticproducer against Staphylococcus aureus and to know the spot of thin layer chromatography showing activity as antibiotic based on bioautography test. Methods. The Actinomycetes (isolate T34) was taken from rhizosphere of Tin (Ficuscarica L.) plant which is grown on Starch Nitrate Agarmedium.The broth culture was prepared using 25 ml of Starch Nitrate Brothmedium having been givena quarter plate of the Actinomycetes isolate and then shaken for 5days. After that, the25 µl of actinomycetesbroth culture was put on the well of plate that had been planted with S. aureus and incubated at 37 °C for 24 hours to measure the inhibition zone diameter.The active broth culture was extracted using ethyl acetate solvent. Components in the extract were analyzed by thin layer chromatography and followed by bioautography process on Mueller Hinton medium having been cultivated with S. aureus for 30 min and incubated for 24 hours. Outcome measured. Inhibition zone diameter against S.aureusgrowth. Results. The results shows that the Actinomycetes (isolate T 34) can inhibit S. aureus with 24,2 mm of inhibition zone diameter. The result of the thin layer chromatography using silica gel GF254 as stationary phase and n-hexane : ethyl acetate (2:1) as mobile phase shows that one spot appears by UV 254 detection and two spots appear by UV 366. One of spots with Rf of 0.56 shows activity as antibiotic. Conclusion. Actinomycetes (isolate T 34)produces antibiotic against S. aureus