Correspondance: bryja@brno.cas.czInternational audienceAnalysis of a single strand conformation polymorphism (SSCP) using capillary electrophoresis (CE) is a novel method to study polymorphism of DNA sequences in large scale population studies. We optimized CE-SSCP analysis to study the major histocompatibility complex (MHC) class II alpha gene (DQA) polymorphism. Short-chain linear polyacrylamide (6%) as sieving matrix, TrisCl (pH 8.5) as buffer for sample dilution, and 27 ° C, 9 kV as electrophoresis parameters were suitable for sufficient resolution of all alleles. We found that almost 25% of clones contained a PCR (polymerase chain reaction) artefact and strict criteria have to be applied when using cloning and sequencing to analyse the...
Clonality analysis is a critical tool for the diagnosis of suspect lymphoproliferative disorders. Am...
Effect of polymer matrix and glycerol on rapid single-strand conformation polymorphism analysis by c...
We describe an effective method of microchip electrophoresis (ME) based on single strand conformatio...
DoctorFor accurate genetic analysis, sequence-based detection of genetic materials should be perform...
International audienceMajor histocompatibility complex (MHC) genes play important role in host-paras...
The use of capillary electrophoresis (CE) in a polymer network for single-strand conformation polymo...
Summary: We examined the influence of electrophoretic conditions on the detectability of small seque...
The multiplex SNaPshot and the capillary electrophoresis-single-strand conformation polymorphism (CE...
Capillary electrophoresis is becoming more and more important in nucleic acid analysis including DNA...
Single-stranded conformation polymorphism (SSCP) by capillary electrophoresis was assessed as a scre...
CE fractions may also be collected and then subjected to additional analysis. Nanoliter fractions co...
Single-strand conformation polymorphism (SSCP) analysis of the second exon of the DQB gene in the en...
BGPI : équipe 6 et 7International audienceThe multiplex SNaPshot and the capillary electrophoresis-s...
Genetic fingerprinting techniques for microbial community analysis have evolved over the last decade...
Genetic variation in the glycoprotein B (gB) gene may play a role in human cytomegaloviruses (HCMVs)...
Clonality analysis is a critical tool for the diagnosis of suspect lymphoproliferative disorders. Am...
Effect of polymer matrix and glycerol on rapid single-strand conformation polymorphism analysis by c...
We describe an effective method of microchip electrophoresis (ME) based on single strand conformatio...
DoctorFor accurate genetic analysis, sequence-based detection of genetic materials should be perform...
International audienceMajor histocompatibility complex (MHC) genes play important role in host-paras...
The use of capillary electrophoresis (CE) in a polymer network for single-strand conformation polymo...
Summary: We examined the influence of electrophoretic conditions on the detectability of small seque...
The multiplex SNaPshot and the capillary electrophoresis-single-strand conformation polymorphism (CE...
Capillary electrophoresis is becoming more and more important in nucleic acid analysis including DNA...
Single-stranded conformation polymorphism (SSCP) by capillary electrophoresis was assessed as a scre...
CE fractions may also be collected and then subjected to additional analysis. Nanoliter fractions co...
Single-strand conformation polymorphism (SSCP) analysis of the second exon of the DQB gene in the en...
BGPI : équipe 6 et 7International audienceThe multiplex SNaPshot and the capillary electrophoresis-s...
Genetic fingerprinting techniques for microbial community analysis have evolved over the last decade...
Genetic variation in the glycoprotein B (gB) gene may play a role in human cytomegaloviruses (HCMVs)...
Clonality analysis is a critical tool for the diagnosis of suspect lymphoproliferative disorders. Am...
Effect of polymer matrix and glycerol on rapid single-strand conformation polymorphism analysis by c...
We describe an effective method of microchip electrophoresis (ME) based on single strand conformatio...