Investigations concerning the cellular origin of dopamine receptors

The cellular investigation of dopamine receptors was examined using glial cell fractions and synaptic fractions prepared from bovine caudate. Binding of the dopamine agonist apomorphine and antagonist spiroperidol was examined. A fractionation of bovine substantia nigra was developed. No evidence of haloperidol binding was found in neuronal, glial or synaptosomal fractions from this tissue. Finally, an autoradiographic localication of [3H]-haloperidol was undertaken. The results support the astroglial localization of a significant portion of the dopamine receptors