Laser microdissection-mediated isolation and in vitro transcriptional amplification of plant RNA

Protocols for laser microdissection and linear amplification of RNA from fixed, sectioned plant tissues are described. When combined with quantitative RT-PCR, microarray analysis, or RNA-sequencing, these procedures enable quantitative analyses of transcript accumulation from microscopic quantities of specific plant organs, tissues, or single cells. © 2009 by John Wiley & Sons, Inc