Multi-platform next-generation sequencing of the domestic Turkey (Meleagris gallopavo): Genome assembly and analysis
- Dalloul, R. A.
- Long, J. A.
- Zimin, A. V.
- Aslam, L.
- Beal, K.
- Blomberg, L. A.
- Bouffard, P.
- Burt, D. W.
- Crasta, O.
- Crooijmans, R. P. M. A.
- Cooper, K.
- Coulombe, R. A.
- De, S.
- Delany, M. E.
- Dodgson, J. B.
- Dong, J. J.
- Evans, C.
- Frederickson, K. M.
- Flicek, P.
- Florea, L.
- Folkerts, O.
- Groenen, M. A. M.
- Harkins, T. T.
- Herrero, J.
- Hoffmann, S.
- Megens, H. J.
- Jiang, A.
- de Jong, P.
- Kaiser, P.
- Kim, H.
- Kim, K. W.
- Kim, S.
- Langenberger, D.
- Lee, M. K.
- Lee, T.
- Mane, S.
- Marcais, G.
- Marz, M.
- McElroy, A. P.
- Modise, T.
- Nefedov, M.
- Notredame, C.
- Paton, I. R.
- Payne, W. S.
- Pertea, G.
- Prickett, D.
- Puiu, D.
- Qioa, D.
- Raineri, E.
- Ruffier, M.
- Salzberg, S. L.
- Schatz, M. C.
- Scheuring, C.
- Schmidt, C. J.
- Schroeder, S.
- Searle, S. M. J.
- Smith, E. J.
- Smith, J.
- Sonstegard, T. S.
- Stadler, P. F.
- Tafer, H.
- Tu, Z.
- van Tassell, C. P.
- Vilella, A. J.
- Williams, K. P.
- Yorke, J. A.
- Zhang, L.
- Zhang, H. B.
- Zhang, X.
- Zhang, Y.
- Reed, K. M.
DOIAbstract
A synergistic combination of two next-generation sequencing platforms with a detailed comparative BAC physical contig map provided a cost-effective assembly of the genome sequence of the domestic turkey (Meleagris gallopavo). Heterozygosity of the sequenced source genome allowed discovery of more than 600,000 high quality single nucleotide variants. Despite this heterozygosity, the current genome assembly (~1.1 Gb) includes 917 Mb of sequence assigned to specific turkey chromosomes. Annotation identified nearly 16,000 genes, with 15,093 recognized as protein coding and 611 as non-coding RNA genes. Comparative analysis of the turkey, chicken, and zebra finch genomes, and comparing avian to mammalian species, supports the characteristic stabi...
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