Tiger Prawn (Penaeus monodon) broodstock of Andaman Molecular Characterisation by RAPD Technique

Random amplified polymorphic DNA (RAPD) analysis was used to examine genetic variation in Tiger prawn, Penaeus monodon. Specimens of this prawn were collected from two different geographically separated locations in the Andaman Sea. A total of 30 samples of P. monodon individuals were investigated using nine random primers. The PCR amplification of template DNA produced a total of 26 scorable RAPD bands, having molecular weight ranging from approximately 500 to 5,000 bp of the nine primers tested, the maximum number of loci amplified was 5 with primer 3 and primer 4 followed by primer 1 which recorded 4 distinct scorable bands