Histochemical procedures for characterizing the crustacean yolk

Histochemical studies on yolk formation in crustaceans not only reveal the chemical nature of various yolk substances but also provide information on the temporal pattern of yolk accumulation and their spatial distribution. In crustaceans the accumulation of yolk material starts with the dispersion of nucleolar extrusions in the ooplasm. The yolk protein to be detected first in the ooplasm is in general glyeolipoprotein in nature. This is followed by the accumulation of a glyeolipoprotein substance that is presumed to be originating from extra-owftaa sources