Induced triploidy in the edible oyster, Crassostrea madrasensis by temperature shock

Heat and cold shock were applied for triploid induction in the edible oyster Crassostrea madrasensis. Triploidy was confirmed by metaphase-spread preparation. The highest triploid induction by cold shock (5°C: 10 min) was 42.2% at larval stage and 33.3% at 'D' stage. With heat shock at 35°C (5 min) triploid percentage was 42% during larval stage and 39.1% at 'D' stage