Expression, purification and antimicrobial activity of recombinant pediocin PA-1 M31L, a PA-1 derivative with enhanced stability
- Kuniyoshi, Taís Mayumi
- O’ Connor, Paula M.
- Arbulu, Sara
- Mesa-Pereira, Beatriz
- Pinheiro de Souza Oliveira, Ricardo
- Hill, Collin
- Ross, Paul
- Cotter, Paul D.
Publication date
March 2019
DOI Publisher
Microbiology Society Journal
Access Microbiology Language
EnglishAbstract
Meeting presentationPediocin, the prototypical class IIa bacteriocin, is an efficient antilisterial molecule. Loss of pediocin PA-1 activity is attributed to methionine oxidation at position 31 and this can be overcome by substituting methionine for leucine (pediocin M31L). The aim of this study was to produce pediocin M31L with enhanced stability by recombinant expression in E. coli cells