The influence of aggregate size fraction and horizon position on microbial community composition
- Fox, Aaron
- Ikoyi, Israel
- Torres-Sallan, Gemma
- Lanigan, Gary
- Schmalenberger, Achim
- Wakelin, Steve
- Creamer, Rachel
DOIAbstract
peer-reviewedThe influence of horizon position and aggregate size on bacterial and fungal community composition was determined. From nine sites, soils were collected from the top three horizon positions (H1, H2 and H3). Physical fractionation separated samples into large macroaggregate (LM, >2000 μm), macroaggregate (MAC, >250 μm), microaggregate (MIC, <250 μm), and silt and clay (SC, 53 μm) fractions. In all samples, the structure of the bacterial and fungal community composition was assessed via restriction fragment length polymorphism (T-RFLP), and for the four aggregate sizes from the top two horizons positions an in-depth analysis of the bacterial community was conducted using next generation sequencing (NGS). Bacterial and fungal comm...
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