Regulation Of High-affinity Branched Chain Amino Acid Transport In Escherichia Coli (repressor, Dna-binding Protein, Sequence, Leucine Regulation).

The genes for branched-chain amino-acid transport in Escherichia coli have been cloned into plasmids and the transport genes order, organization and DNA sequences have been determined. The expression of these genes is regulated by leucine primarily through a trans-acting negative controlling element. This dissertation presents the cloning of the regulatory gene and the identification of it by complementation analysis with strains of bacteria carrying mutations in transport regulation. The DNA sequence of the gene was determined and the calculated MW of the regulatory product is 19,155. The cloned regulatory gene has been transformed into E. coli minicells and the regulatory protein is strongly expressed in vivo. The secondary structure prediction for the regulatory protein indicates that the carboxy terminus may fold into several alpha helices. Two of these predicted alpha helices show sequence homology to DNA-binding proteins.PhDBiological SciencesGeneticsMolecular biologyUniversity of Michigan, Horace H. Rackham School of Graduate Studieshttp://deepblue.lib.umich.edu/bitstream/2027.42/127787/2/8600400.pd