Species diversity, antifungal susceptibility and phenotypic and genotypic characterisation of Exophiala spp. infecting patients in different medical centres in Brazil

The Exophiala genus is responsible for many superficial and invasive infections resulting from black fungi. Identification of Exophiala at the species level is based on morphological observations complemented by molecular tests. The aim of this study was to identify 23 clinical isolates of Exophiala spp. and evaluate the antifungal susceptibility to seven different agents. Molecular identification was based on an analysis of ITS region of rDNA using genomic databases. The micromorphology was evaluated by microculture and scanning electron microscopy. The susceptibility tests were performed using the antifungal agents 5-fluorocytosine (5-FC), amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), posaconazole (PSC), caspofungin (CFG) and terbinafine (TRB). The ITS analysis identified 100% of the following isolates as: E.dermatitidis (8), E.xenobiotica (6), E.bergeri (4), E.oligosperma (3), E.spinifera (1) and E.mesophila (1). The antifungal susceptibility tests showed that the triazoles compounds were in vitro the most active agents against Exophiala. ITS sequencing enabled the accurate identification of the 23 tested isolates. The triazoles, particularly itraconazole and posaconazole, exhibited MIC values lower than AMB, CAS and 5-FC. Although the guidelines do not indicate AMB for treatment against Exophiala spp., this study showed activity for all of the tested species, except E.mesophila.Coordenacao de Aperfeicoamento de Pessoal em Nivel SuperiorCAPESUniv Fed Sao Paulo, Lab Especial Micol, Disciplina Infectol, Sao Paulo, BrazilFundacao Oswaldo Ramos, Hosp Rim, Sao Paulo, BrazilUniv Fed Espirito Santo, CIMM, Dept Patol, Vitoria, BrazilUniv Fed Alfenas, Inst Ciencias Biomed, Dept Microbiol & Imunol, Alfenas, BrazilUniv Fed Sao Paulo, Lab Especial Micol, Disciplina Infectol, Sao Paulo, BrazilCAPES: PNPD 23038.007393/2011-11Web of Scienc