Theory of Enzymatic Reactions in Cyclic Square-Wave Voltammetry: MATHCAD Simulation Protocol

Protein-film voltammetry is seen as simplest methodology designed to study electrochemical features of lipophilic redox enzymes. By adsorbing a given redox enzyme on working electrode surface, it is possible to recognize the mechanism of action of many important proteins. Moreover, one can get access to relevant thermodynamic and kinetic parameters, which might reveal important chemical and physiological aspects of many enzyme-substrate interactions. Understanding the electrochemical behavior of redox enzymes contributes to a better understanding of many metabolisms in living systems. In addition, it also brings important information for designing of specific biosensors, simple medical devices, and bio-fuel cells. In this work, we present a working protocol in MATHCAD to simulate enzymatic reactions of lipophilic redox enzymes in cyclic square-wave voltammetry. This is a novel technique that is a hybrid between the cyclic and the square-wave voltammetry, especially suitable to evaluate mechanisms, kinetics and thermodynamics of surface confined molecules. We present the readers a full MATHCAD file for simulation of these reaction mechanism in Cyclic square-wave voltammetry. This MATHCAD FILE will be of help to experimentalists working in area of enzymatic voltammetry and surface redox mechanisms