Determination of cannabidiol and Δ9tetrahydrocannabinol in Cannabis sativa L. preparations present

The aim of this study was to apply in-house HPLC/DAD method on the Cannabis sativa L. preparations present in the European market and to control their quality. The satisfactory chromatographic data proved that our in-house gradient HPLC/DAD method can be successfully used for determination of the active substance CBD and traces of THC in Cannabis sativa L. preparations. The results obtained for the analyzed samples of Cannabis sativa L. preparations showed higher amounts of CBD than declared value in the certificate (differences ranged from 9.7% m/m to 25.4% m/m). In two samples, the amount of THC found was under allowed maximum limit of 0.20%, while in three samples the amount was above that value. Not all the samples tested have composition as declared in the certificate, although they all origin from a European manufacturer and are present in the European market