Patogénne mikroorganizmy v surovom mlieku

This thesis analyses the occurrence of the pathogenic microorganisms in raw milk. The theoretical part of this thesis focuses on the description of the particular microorganisms, their biochemical activity, their source and the transmission. Furthermore, this thesis analyses the diseases related to these particular microorganisms. In addition, this study discusses a legislation of the raw milk distribution and trade with a focus on its benefits and threats for the final consumers. The practical part includes an analysis of the total number of microorganisms, psychrotrophic bacteria, bacteria Salmonella spp., bacteria Listeria monocytogenes, Staphylococcus aureus and Escherichia coli in raw milk. The samples for this study were collected from the milk automats in the South Moravian region and from the farm in the Pardubice region. Samples were collected during the following year periods: autumn/winter, spring/summer. The analysis includes a comparison of the samples with the legislative limits. Furthermore, the samples were compared with each other in according to the particular period of the year. The last stage of the analysis determines the presence of the inhibitory substances in raw milk