Aplikace molekulárních markerů u pšenice s nestandardním zbarvením obilky

In recent decades the interest of wheat with non-standard discoloration of caryopsis has increased, because of its positive impacts on health. The pigments anthocyanins, which are included in the protective layer of grain, have antioxidant effects. A group of genotypes from Agrotest fyto Ltd. Kroměříž was chosen which consisted of eighteen samples of wheat with non-standard discoloration of caryopsis. Fourteen samples were blue (aleuron), tree yellow (endosperm) and one sample had a red (pericarp). By these samples PCR-based analyses were performed to detect the Waxy gene zero allele, the Glu-A3 glutenin locus and the hardness of grain by puroindoline genes. These parameters are significant indicators of wheat quality. By all genotypes the presence of allele Pina-D1a/b was detected and by a few the allele Pinb-D1a/c/d. These detected alleles, which are included in the analyzed wheat, should be sorted to the hardness wheats because they are suitable for baking purposes. The identified material (V1 104 15), a so-called waxy genotype, was positive in the presence of all three zero alleles in the Waxy gene. Similar lines were used in Asia for the preparation of pastas. At the locus Glu-A3, the Glu-A3d allele was detected in all tested genotypes. Along with this, Glu-A3f was founded in some genotypes. These alleles have a positive effect on dough quality, especially on ductility. The results will be used for further breeding work