Vliv selenu na růst heterotrofní formy řasy Chlorella

This diploma thesis (Heterotrophic incorporation of selenium in Chlorella sp.) focuses on characterisation of the genus Chlorella and large-scale use of algae for biofuel and starch production. Besides that, the thesis contains an up-to-date summary of forms of autotrophic algae cultivation ranging from large-volume tanks covering the area of dozens of hectares down to laboratory cultivation in photobioreactors and heterotrophic cultivation of algae in fermenters. Experimental part of the thesis focuses on cultivation and observation of the heterotrophic growth of algae Chlorella vulgaris in relation to the concentration of added selenium which was performed on glucose substrate with varying concentrations and forms of selenium. Selenium was applied in a form of sodium selenite Na2SeO3 and sodium selenate Na2SeO4. Numerous experimental repetitions suggest a significantly important effect of the form of the added selenium on growth rate and vitality of Chlorella sp. While the algae did not thrive on sodium selenite medium, they prospered on sodium selenate and exhibited faster growth compared to that on the media without additional selenium