Hodnocení kvality ejakulátu u ovčáckých plemen psů

This diploma thesis deals with the quality of ejaculate of shepherd dog breeds. In the first part we will classify shephed dogs within the FCI organization and we will approach the breeds that we assess in this work. We will describe the anatomical and physiological characteristics of the reproductive organ of male dog, its ejaculate, ejaculation, semen and their development. We will also learn about the possibilities of dog ejaculate collection. In the main section we will deal with the evaluation of the ejaculate and we will focus on the macroscopic and microscopic examination of fresh ejaculate. In the course of the work, 100 ejaculates from 28 dogs belonging to I. FCI group will be evaluated, each dog will be taken twice to four times and one shot will be made from each sampling. The dogs will be divided into groups according to age, breed, stabling and feeding, and the examined parameters of the quality of the ejaculate will be discussed and compared. In terms of the monitored indicators, a highly statistically significant effect (P is less than or equal 0.01) of the order of collection on the volume of ejaculate obtained will be demonstrated. A statistically significant difference (P is less than or equal 0.05) between the first and the second sampling will be found in the evaluation of the effect of the order of collection during one day. The second volume will be found to be lower than the first one. It will be also shown that this value is affected by the age of the dog. According to our research, breed type and age of the dog causes a statistically significant difference (P is less than or equal 0,05) for sperm activity. The order of collection and stabling have a lesser influence on this indicator and the feed represents a negligible factor. Concerning the sperm concentration in the ejaculate, we will demonstrate a statistically significant difference (P is less than or equal 0.01) between the age groups of the dogs and the type of their accommodation. We will find a statistically significant difference (P is less than or equal 0.05) in the order of sampling. The factors of breed type and feed will have minimal influence in this case. We will not prove a statistical difference in the influence of the studied factors on color and odor, and we will be able to assert that these factors have negligible influence on these values. As for the qualitative sperm indicators, we will focus on the percentage of altered heads, connecting parts, flagella, degenerate and immature sperm. Concerning the values, we will conclude that the breed type, the age and, in the case of immature sperm, the order of collection have the most important influence on these parameters