Stabilita membrán jako měřítko odolnosti vůči chladovému stresu u kontrastních ekotypů Arabidopsis thaliana

Cold acclimation is a process of adaptation to suboptimal temperatures preparing the plants to efficiently reduce the upcoming damage that may be caused by sub-zero temperature. The early phase of cold acclimation is associated with stimulation of the protective mechanisms and fast suppression of growth. This work was focused on the comparison of the effect of normal and low light intensity on the stability of the membrane in Arabidopsis plants during the cold acclimation period. Ion conduc-tivity is a sensitive method that directly evaluates the impact of stress on plant fitness and thus, we used the electrolyte leakage method for assessing cell membrane stability to test the cold stress tolerance. Next, we will focus on the natural variation of stress responses in a broad spectrum of Arabidopsis ecotypes to dissect genetic traits confer-ring the plants with a tolerance to cold and freezing stress. During the monitoring of expression profiles of the cold signaling pathway genes for various resistant ecotypes it was found, that ecotype C24 is sensitive to low temperatures, although it shows sig-nificant tolerance to other stresses. The Tamm-2 ecotype, conversely, shows a high level of cold and frost tolerance and has a high expression of cold signaling genes. Cold tol-erance correlated with expression of genes that contribute to low temperature responses: CBF1, CBF2, CBF3, and COR47