Polymorfismus genu CYP2E1 ve vztahu k hladině skatolu a androstenonu kanců

This thesis discusses how polymorphism in this gene can affect the metabolism of the skatole and androstenone in boar's livers. The accumulation of these substances in adipose tissue causes an undesirable odor of entire male meat which negatively affects the use of boars in fattening. Using selection of individuals with CYP2E1 polymorphisms which provably reduce skatole levels in adipose tissue could be a solution that would not affect animal welfare and could improve meat quality and breeding economy. The DNA from 90 samples of gilts, entire male pigs and castrated male pigs blood was isolated. These samples were sequenced by the Sanger sequencing method to determine the frequency of alleles and genotypes of given polymorphisms. There was association analysis of observed genotypes and measured values of skatole and androstenone performed. Due to the low quantity of samples with higher measured values of boar taint components, no statistically significant differences were found between genotypes and these traits