Studium bílkovinného komplexu zrna pšenice a ječmene po aplikaci dusíku a síry

The thesis objective was to check out the effect of nitrogen fertilizers and nitrogen fertilizers with sulphur in nutrition of spring barley (variety Bojos) and winter wheat (variety Mulan). The quality of protein complex was determined on grain samples by the HPLC method. The application of sulphur showed also in the fractional composition of the proteinous complex. The wheat grain without applied sulphur fertilizers had significantly higher percentage proportion of ?-gliadins, albumins and globulins compared to the variants treated this way. It was at the expense of gluten proteins. The greatest fertilization effect was proved after application of Yara Vita Thiotrac in late vegetation period. The proportion of barley´s LMW glutelins, D-hordeins, albumins and globulins has demonstrably increased in the variants with applied sulphur. The changes of fractional protein composition caused by degradation of high-molecular compounds during malting and mashing were confirmed