Analytical method development and its validation for simultaneous estimation of catechin and curcumin by HPTLC from ancho lean tablets

HPTLC method which is simple, particular and robust has been developed for the simultaneous estimation of Catechin and Curcumin from an Ayurvedic formulation. The method was validated using parameters such as linearity, specificity, and precision, limit of quantification (LOQ), limit of detection (LOD), accuracy and robustness as per ICH guidelines. The present work deals with development of HPTLC method for simultaneous estimation of catechin and curcumin in marketed Ayurvedic formulations. Chromatographic separation of the drugs was performed on Merck TLC aluminium plates pre-coated with silica gel 60F254 as the stationary phase. The mobile phase selected was toluene:ethyl acetate: formic acid (7: 2.5: 0.5 v/v/v). The sample solutions were prepared in methanol and linear ascending development was carried out in twin trough glass chamber and scanned at 269 nm using Camag TLC scanner. The two markers were resolved successfully with Rf values 0.23±0.02 and 0.58±0.02 for catechin and curcumin, respectively. The regression analysis data indicated good linear relationship for the calibration plots for Catechin and Curcumin in the range of 1900-2500 ng/spot and 200-800 ng/spot and regression coefficient was 0.990 and 0.997 respectively. The proposed method can be used for the estimation of these markers in combined Ayurvedic formulation