Tetrazolium test in Copaifera langsdorffii and Schizolobium parahyba seeds

Este trabalho objetivou padronizar o teste de tetrazólio para avaliação da viabilidade de sementes de copaíba e guapuruvu. Avaliou as seguintes metodologias: sementes escarificadas mecanicamente e embebidas por 24 e 48 horas, com posterior retirada do tegumento e imersas em solução de tetrazólio a 0,075, 0,10 e 0,20% por 2, 3 e 4 horas, a 35 ºC, no escuro. As metodologias avaliadas que foram eficientes na obtenção de coloração satisfatória, permitindo a diferenciação dos tecidos, e na avaliação da qualidade fisiológica das sementes, quando comparadas com o teste padrão de germinação, foram: para a espécie copaíba, sementes escarificadas e embebidas por 24 horas, a 35 ºC, com posterior retirada do tegumento, submetidas a solução de tetrazólio a 0,20% por 4 horas, a 35 ºC, no escuro; e para a espécie guapuruvu, sementes escarificadas e embebidas por 48 horas, a 35 ºC, com posterior retirada do tegumento, submetidas a solução de tetrazólio a 0,10% por 4 horas, a 35 ºC, no escuro.Palavras-chave: Sementes florestais; teste rápido; copaíba; guapuruvu. AbstractTetrazolium test in Copaifera langsdorffii and Schizolobium parahyba seeds. This research aimed to standardize the tetrazolium test for evaluation of viability of Copaifera langsdorffii Desf. and Schizolobium parahyba Vell. Blake seeds. It evaluated the following methodologies: seeds scarificated mechanically and soaked up by 24 and 48 hours, with posterior seed coat removal and immersed in tetrazolium solution at 0.075; 0.10 and 0.20% for 2, 3 and 4 hours, 35 ºC, the dark one. The evaluated methodologies that had been efficient in the attainment of satisfactory coloration, allowing the differentiation of tissues, and in the evaluation of the physiological quality of the seeds when compared with the germination test, had been: for the species Copaifera langsdorffii, seeds scarificated and soaked up by 24 hours, 35 ºC, with posterior seed coat removal, submitted to the tetrazolium solution 0.20% for 4 hours, 35 ºC, in the dark one, and for the species guapuruvu, seeds scarificated and soaked up by 48 hours, 35 ºC, with posterior seed coat removal, submitted to the tetrazolium solution 0.10% for 4 hours, 35 ºC, in the dark one.Keywords: Forest seeds; fast test; Copaifera langsdorffii; Schizolobium parahyba.This research aimed to standardize the tetrazolium test for evaluation of viability of Copaifera langsdorffii Desf. and Schizolobium parahyba Vell. Blake seeds. It evaluated the following methodologies: seeds scarificated mechanically and soaked up by 24 and 48 hours, with posterior seed coat removal and immersed in tetrazolium solution at 0.075; 0.10 and 0.20% for 2, 3 and 4 hours, 35 ºC, the dark one. The evaluated methodologies that had been efficient in the attainment of satisfactory coloration, allowing the differentiation of tissues, and in the evaluation of the physiological quality of the seeds when compared with the germination test, had been: for the species Copaifera langsdorffii, seeds scarificated and soaked up by 24 hours, 35 ºC, with posterior seed coat removal, submitted to the tetrazolium solution 0.20% for 4 hours, 35 ºC, in the dark one, and for the species guapuruvu, seeds scarificated and soaked up by 48 hours, 35 ºC, with posterior seed coat removal, submitted to the tetrazolium solution 0.10% for 4 hours, 35 ºC, in the dark one