PENGARUH FORMALIN DAN pH TERHADAP DAYA HIDUP SPERMATOZOA SAPI YANG DISIMPAN PADA SUHU RUANGAN MAUPUN 5°C

The study was conducted in Research Institute for Animal Production (RIAP)-Ciawi. Semen was collected from 3 bulls twice a week by artificial vagina (AV), diluted in Tris buffer (control) and containing of 0.0625% formalin, reducing pH to 5.5 or their combination to temporally inhibit the motility of sperm.Diluted semen were store at ambient temperature for 0, 3 and 24 hours or at 5°C for 0, 3 and 7 days.Reactivation of motility was performed by dilution of formalin and bring the pH to 7.0. In ambient temperature,formalin and pH significantly (p<0.01) inhibit the motility of sperm, but these inhibition was reversible. The percentage of motility was significantly higher(p