Potential Of Endophytic Fungi Isolates From Vetiver (Vetiveriazizanioides Linn) As A Biolarvacide for Aedesaegypti L.

Abstract. Control of vector that causes Dengue Hemorrhagic Fever (DHF) isstill reliable because until now there has not been found a cure for DHF. Theexistence of negative impacts resulting from the use of chemical pesticidescauses the need to find alternatives to larvacides that are more environmentallyfriendly. Larvacides that have been developed are utilizing biological larvacides(biolarvacides). One organism that has potential as a larvacide is endophyticfungi. This is based on the probability that the endophytic fungi that remain inplants have the ability to synthesize the same compounds as the host plant.Therefore, it is necessary to conduct research to determine the potential ofsecondary metabolites of endophytic fungi isolates from vetiver (V. zizaniodes)as biolarvacidesfor Aedesaegypti. Secondary metabolites were extracted frompure endophytic fungi isolates (IH 8, IH 19 and IH 21). Larvicidal potential testagainst A. aegypti was carried out with a Completely Randomized Design with3 replications. The concentrations of the crude extracts of secondary metabolitesused were 2%, 4%, 8% and 16% and 0% as controls. Then the percentage oflarvae, pupae and adult that died in each treatment was calculated. Observationswere done for 8 days. The results showed that secondary metabolites of the threeisolates have the potential to cause biolarvacidal for A. aegypti. The resultsshowed that secondary metabolites of the 3 isolates had the potential asbiolarvacides for A. aegypti. The effects include accelerating the developmentof larvae into pupae, causing pupae death, death of young adults and abnormaladults. The treatment with IH 08 and IH 21at a concentration of 8% and 16%,larvae death has begun to occur on the first day