Comparison of Antioxidant and Anti-collagenase Activities Ethanol Extract of Black Soybeans with Daidzein Compounds

Aging is a natural process in human life; one of the triggers is free radicals. The use of antioxidants from natural ingredients is an effort to overcome premature aging and counteract the harmful effects caused by free radicals. Black soybean (Glycine soja), which easily obtained in Indonesia, contains a nutritional value that is very good for human health. The protein content is quite high (40.4 g / 100g) and rich in antioxidants. The results presented in this study revealed that black soybean extract (Glycine max (L.) Merr.) Had a higher antioxidant activity of FRAP compared to Daidzein compounds, black soybean extract had antiaging activity through better collagenase inhibition compared to Daidzein compounds. The largest FRAP activity at 50 ?g / mL black soybean extract concentration was 146.35 ?M Fe (II) / ?g) and Daidzein 50 ?g / mL concentration was 96.93 (?M Fe (II) / ?g). Anticolagenase activity based on the IC50 value of black soybean extract was 84.73 ?g / mL, and Daidzein was 98.18 ?g / mL. From the above results, it can conclude that ethanol extract of black soybeans has excellent potential as an antioxidant that is useful as inhibiting collagenase