Identification of Ketoprofen in Drug Formulation and Spiked Urine Samples by Micellar Thin Layer Chromatography and its Quantitative Estimation by High Performance Liquid Chromatography

A simple, selective and economical micellar thin layer chromatographic method for on-plate identification of ketoprofen from pure, pure, formulated and spiked urine samples was developed. The proposed method involves use of amino acid impregnated silica gel layers as stationary phase with mixed micelles (0.5% aqueous solutions of sodium dodecyl sulphate plus Triton X-100 and acetone (8:5:1.5, v/v) as mobile phase. The nature as well as the concentration of surfactant influences the mobility of ketoprofen. The interference study was carried out using various organic and inorganic metabolites, usually found in human urine. The HPLC determination of ketoprofen (formulated and spiked urine) samples carried out at l=270 nm with mobile phase comprising of acetonitrile: double distilled water: acetic acid (1:1:1, v/v). The correlation coefficient was 0.99 and the recoveries of ketoprofen (formulated and spiked urine) were within range of 94.0-100.2% with relative standard deviation ranging from 0.6-0.86%