Effect of fruit essential oil on ephedrine induced manic like behavior: evidence from a new protocol

Introduction: Estimating ephedrine’s effect on animal’s locomotor activity and evaluation of cumin fruit essential oil (FEO) effect and mechanism of ephedrine-induced hyper-locomotion.Methods: A new protocol developed for evaluation of manic behavior induced by ephedrine in a dose and time dependent manner. Following the suppressive effects of cumin FEO on acquisition and expression of ephedrine induced manic behavior was examined. Furthermore, the effect of L-NAME on expression of manic behavior and effect of bicuculline on suppressive effects of cumin FEO was evaluated.Results: Ephedrine at the dose of 100 mg/kg (i.p.) in a five days protocol significantly increased the locomotor activity in mice. In addition, cumin FEO at the specific dose of 2% significantly suppressed the acquisition and expression of hyperactivity induced by ephedrine. L-NAME at doses of 30 (p<0.01) and 40 mg/kg (p<0.05) showed similar effects as cumin FEO at the dose of 2%. Bicuculline (as a GABAA antagonist) at the specific dose of 2 mg/kg (p<0.01) could significantly reverse the suppressive effects of cumin at the dose of 2%.Conclusion: It could be concluded that cumin can suppress the manic behavior induced by ephedrine through the both previously suggested mechanisms of nitric oxide syntheses pathway and GABAergic system.Declaration of Interest:None.Keywords: Cuminum cyminum fruit, Ephedrine, Manic disorder