Effects of Leucaena leucocephala Supplementation to Total Mixed Ration Based on Ammoniated Rice Straw Onfiber Digestibility and Rumen Fermentation Characteristics in vitro

Legume is a feed ingredient that contains high protein for ruminants. The tannin content in legumes can provide a bypass protein for ruminants. This study was done to find out the effect of legume supplementation (Leucaena leucocephala) on fiber digestibility, characteristic rumen, protozoa population and methane production from total mixed ration (TMR) based on ammoniated rice straw. The study was conducted in vitro using a randomized block design with 3 treatments and 5 replications. The treatment was A. 40% ammoniated rice straw + 60% concentrate, B. 40% ammoniated rice straw + 50% concentrate + 10% L. leucocephala, C. 40% ammoniated rice straw + 40% concentrate +10, + 20% L.leucocephala.The results showed that the addition of L. leucocephala increased digestibility of NDF, ADF, and Cellulose, RUP (rumen undegradable protein) and reduced protozoa population and methane production (p <0.05). Increased doses of L.leucocephala up to 20% reduce fiber digestibility of feed substances compared to a dose of 10% but are still higher than controls. The results of this study concluded that L.leucocephala supplementation in TMR based on ammoniated rice straw in vitro improved digestibility, fermentability, and reduced methane gas production. Supplementation of 10% and 20% L. leucocephala needs further research, to see the effect on livestock in vivo