The present study was carried out to evaluate the effects of different activation protocols, enucleation methods, and culture media on the development of parthenogenetic and nuclear transfer (NT) rabbit embryos. Electroporation of 25 mM inositol 1,4,5-trisphosphate (IP3) in calcium- and magnesium-free PBS immediately induced a single intracellular calcium transient in 6 out of 14 metaphase II-stage rabbit oocytes evaluated during a 10-min recording period. The percentage of oocytes treated with IP3 followed by 6-dimethylaminopurine (IP3 + DMAP) that cleaved (83.9%) and reached the blastocyst stage (50%) was significantly higher (p \u3c 0.05) than those activated with multiple pulses (61.6% and 30.1%, respectively) or treated with ionomycin ...
Since the crucial step in successful nuclear transfer protocols is the activation of recipient oocyt...
925-934Pluripotent stem cells are possibly the best candidates for regenerative medicine, and somat...
The viability of SCNT embryos is poor, with an extremely low cloned piglet production rate. In the p...
The present study was carried out to evaluate the effects of different activation protocols, enuclea...
This study evaluated procedures to improve nuclear transplantation efficiency in rabbit embryos. Rec...
The rabbit was used as a model for nuclear transfer. A critical step in nuclear transfer is oocyte a...
The first six genetically verified nuclear transplant rabbits have been produced in this study. Indi...
This study was designed to examine the effect of the age of rabbit oocytes on the developmental pote...
The preimplantation period in the rabbit consists of a 3 day cleavage stage during which the number ...
A technique for successfully freezing, thawing and transferring rabbit embryos has been developed. M...
The information about reproductive biology, especially embryo development of Asian elephant (Elephas...
The functional interrogation of factors underlying early mammalian development is necessary for the ...
The functional interrogation of factors underlying early mammalian development is necessary for the ...
This study aimed to verify the beneficial effect of cycloheximide (CHX) treatment on the development...
The most successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos has be...
Since the crucial step in successful nuclear transfer protocols is the activation of recipient oocyt...
925-934Pluripotent stem cells are possibly the best candidates for regenerative medicine, and somat...
The viability of SCNT embryos is poor, with an extremely low cloned piglet production rate. In the p...
The present study was carried out to evaluate the effects of different activation protocols, enuclea...
This study evaluated procedures to improve nuclear transplantation efficiency in rabbit embryos. Rec...
The rabbit was used as a model for nuclear transfer. A critical step in nuclear transfer is oocyte a...
The first six genetically verified nuclear transplant rabbits have been produced in this study. Indi...
This study was designed to examine the effect of the age of rabbit oocytes on the developmental pote...
The preimplantation period in the rabbit consists of a 3 day cleavage stage during which the number ...
A technique for successfully freezing, thawing and transferring rabbit embryos has been developed. M...
The information about reproductive biology, especially embryo development of Asian elephant (Elephas...
The functional interrogation of factors underlying early mammalian development is necessary for the ...
The functional interrogation of factors underlying early mammalian development is necessary for the ...
This study aimed to verify the beneficial effect of cycloheximide (CHX) treatment on the development...
The most successful development of interspecies somatic cell nuclear transfer (iSCNT) embryos has be...
Since the crucial step in successful nuclear transfer protocols is the activation of recipient oocyt...
925-934Pluripotent stem cells are possibly the best candidates for regenerative medicine, and somat...
The viability of SCNT embryos is poor, with an extremely low cloned piglet production rate. In the p...