Effect Of Toxoplasma Gondi Profilin Exposure On High Density Lipoprotein (Hdl) Level In Normal And Hypercaloric Diet Induced Rattus Norvegicus Wistar Strain Rats

Toxoplasma gondii, a protozoa that causes toxoplasmosis. It is produces in cats, stray cats and domestic is the definitive host. Profilin is a small actinbinding protein that affects the actin cytoskeleton. and it can be found alone in all eukaryotic kingdoms. Profilin interacts with several molecules such as phosphatidylinositol-4,5-bisphosphate proline-containing proteins, and actinrelated proteins. Via singal transduction this interaction will connect the profilin so that there will be a reorganization of the actin cytoskeleton. It has been known for some time that T. gondii is not capable of synthesizing cholesterol (Chl), and thus depends on uptake of host Chl for its own development. Chl metabolism in mammalian cells involves direct transport of preformed sterol from the liver in the form of very low density lipoprotein (VLDL) and low density lipoprotein (LDL) to the periphery. In fact, it has been shown that T. gondii acquires host Chl via endocytosis mediated by the LDL receptor or the LDL receptor-related protein. This shows that Toxoplasma depends on the host for Chl and uptaking it via LDL receptor. This may explain the fact that, the endocytosis causing the LDL level to increase, directly causing the HDL level to decrease. This study was conducted to determine the effect of Toxoplasma gondii profilin exposure on HDL levels in normal and hypercaloric induced Rattus Norvegicus Strain Wistar rats. The research is a experimental study in the laboratory with true experimental – posttest control-group design only. The experiment was conducted at the Pharmacology Lab and Parasitology Lab of Medical Faculty University of Brawijaya. Result of Kruskal Walis test, (P = 0.252; α = ≥0.05), shows that there were not any significant difference on HDL levels in all groups. The result of correlation coefficient shows the majority of groups, show a weak correlation between Toxoplasma gondii profilin exposure HDL level with negative direction. It can be concluded that, Toxoplasma gondii profilin does not influence the HDL level in Rattus Norvegivus Wistar Strain rats